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JoVE Journal
Developmental Biology

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Establishment of Genome-edited Human Pluripotent Stem Cell Lines
 

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Article DOI: 10.3791/53583
February 2nd, 2016

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Summary February 2nd, 2016

Genome editing of human pluripotent stem cells (hPSCs) can be done quickly and efficiently. Presented here is a robust experimental procedure to genetically engineer hPSCs as exemplified by editing the AAVS1 safe harbor locus to express EGFP and introduce antibiotic resistance.

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