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Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

DOI:
10.3791/53583-v

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09:51 min

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February 02, 2016

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, ,
1Department of Molecular and Cell Biology,University of California, Berkeley

Chapters

  • 00:05Title
  • 00:37Editing Pluripotent Stem Cells
  • 05:00Picking Transfected Colonies
  • 07:54Results: Comparison of EGFP-positive and Targeted TALEN, ZFN and CRISPR/Cas9 Human Stem Cell Colonies
  • 09:00Conclusion

Summary

Automatic Translation

Automatic Translation

Genome editing of human pluripotent stem cells (hPSCs) can be done quickly and efficiently. Presented here is a robust experimental procedure to genetically engineer hPSCs as exemplified by editing the AAVS1 safe harbor locus to express EGFP and introduce antibiotic resistance.

Tags

Keywords: Genome EditingHuman Pluripotent Stem CellsDisease ModelingColony IsolationTargetingTransfectionCRISPRZinc Finger NucleaseTALONFeeder CellsTrypsinizationCell Culture

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