Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biology

A subscription to JoVE is required to view this content.
You will only be able to see the first 2 minutes.

JoVE Journal Biology
Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing
Microbial Communities in Nature and Laboratory - Interview
Journal (Biology)
Microbial Communities in Nature and Laboratory - Interview
Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells
Journal (Biology)
Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells
 

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

Article DOI: 10.3791/53586
October 11th, 2015 •
1, 1
1Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia

Chapters

Summary
Automatic Translation
October 11th, 2015

Here, we present a protocol for specific siRNA-mediated mRNA depletion followed by immunofluorescence analysis to evaluate meiotic spindle assembly and organization in mouse oocytes. This protocol is suitable for in vitro depletion of transcripts and functional assessment of different spindle and/or MTOC-associated factors in oocytes.

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter