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October 11, 2015
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Here, we present a protocol for specific siRNA-mediated mRNA depletion followed by immunofluorescence analysis to evaluate meiotic spindle assembly and organization in mouse oocytes. This protocol is suitable for in vitro depletion of transcripts and functional assessment of different spindle and/or MTOC-associated factors in oocytes.
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Cite this Article
Baumann, C., Viveiros, M. M. Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing. J. Vis. Exp. (104), e53586, doi:10.3791/53586 (2015).
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