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Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing
Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing
JoVE Journal
Biology
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JoVE Journal Biology
Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

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09:16 min

October 11, 2015

DOI:

09:16 min
October 11, 2015

11636 Views
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Summary

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Here, we present a protocol for specific siRNA-mediated mRNA depletion followed by immunofluorescence analysis to evaluate meiotic spindle assembly and organization in mouse oocytes. This protocol is suitable for in vitro depletion of transcripts and functional assessment of different spindle and/or MTOC-associated factors in oocytes.

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