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Biology

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Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing
 

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

Article DOI: 10.3791/53586
October 11th, 2015

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Summary October 11th, 2015

Here, we present a protocol for specific siRNA-mediated mRNA depletion followed by immunofluorescence analysis to evaluate meiotic spindle assembly and organization in mouse oocytes. This protocol is suitable for in vitro depletion of transcripts and functional assessment of different spindle and/or MTOC-associated factors in oocytes.

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