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JoVE Journal
Immunology and Infection

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Ratiometric Imaging of Extracellular pH in Dental Biofilms
 

Ratiometric Imaging of Extracellular pH in Dental Biofilms

Article DOI: 10.3791/53622
March 9th, 2016

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Summary March 9th, 2016

A pH-sensitive ratiometric dye is used in combination with confocal laser scanning microscopy and digital image analysis to monitor extracellular pH in dental biofilms in real-time.

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