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JoVE Journal
Biology

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Identification of Kinesin-1 Cargos Using Fluorescence Microscopy
 

Identification of Kinesin-1 Cargos Using Fluorescence Microscopy

Article DOI: 10.3791/53632
February 14th, 2016

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Summary February 14th, 2016

Here, a protocol is presented to identify Kinesin-1 cargos. A motorless mutant of the Kinesin-1 heavy chain (KIF5B) aggregates in the cytoplasm and induces aggregation of its cargos. Both aggregates are detected under fluorescence microscopy. A similar strategy can be employed to identify cargos of other motor proteins.

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