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JoVE Journal
Biology

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Identifikation af kinesin-1 Cargos Brug Fluorescens Microscopy
 
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Identifikation af kinesin-1 Cargos Brug Fluorescens Microscopy

Article DOI: 10.3791/53632
February 14th, 2016

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Summary February 14th, 2016

Please note that all translations are automatically generated.

Click here for the English version.

Her er en protokol forelægges identificere kinesin-1 ladninger. En motorless mutant af kinesin-1 tung kæde (KIF5B) aggregater i cytoplasmaet og inducerer sammenlægning af sine laster. Begge aggregater opdages under fluorescensmikroskopi. En lignende strategi kan anvendes til at identificere laster af andre motordrevne proteiner.

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