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JoVE Journal
Immunology and Infection

This content is Open Access.

Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain
 

Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain

Article DOI: 10.3791/53658 12:14 min February 12th, 2016
February 12th, 2016

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Summary

Inflammation plays a central role in the pathogenesis of ischemic stroke. Increasing evidence suggests that it acts as a double-edged sword which exacerbates early brain injury, but also contributes to later repair. This protocol describes the isolation of immune cells from the ischemic brain and their subsequent flow cytometric phenotyping.

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Tags

Isolation Flow Cytometric Analysis Immune Cells Ischemic Mouse Brain Quantification Phenotypic Characterization Multiparametric Flow Cytometry Post-ischemic Inflammation Lesion Progression Repair Stroke Outcome Density Gradient Centrifugation Viable Immune Cells Immunomodulatory Treatment Strategies Peristaltic Perfusion Pump Tube Calcium-and Magnesium-free HBSS 23-gauge Needle Dissection Board Plastic Tray Fore And Hind Paws 20-gauge Needles Abdominal Skin Lateral Incision Integument Abdominal Wall Sharp Iris Scissors Liver
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