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Biology

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A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3
 

A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3

Article doi: 10.3791/53723
January 24th, 2016 Usage Statistics

Summary January 24th, 2016

Here we describe a procedure allowing a detailed analysis of the phosphorylation-dependent activation of the IRF3 transcription factor. This is achieved through the combination of a high resolution SDS-PAGE and a native-PAGE coupled to immunoblots using multiple phosphospecific antibodies.

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