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A Magnetic Microbead Occlusion Model to Induce Ocular Hypertension-Dependent Glaucoma in Mice
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JoVE Journal Medicine
A Magnetic Microbead Occlusion Model to Induce Ocular Hypertension-Dependent Glaucoma in Mice

A Magnetic Microbead Occlusion Model to Induce Ocular Hypertension-Dependent Glaucoma in Mice

DOI:
10.3791/53731-v

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09:26 min

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March 23, 2016

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, , ,
1Department of Neuroscience,CRCHUM and University of Montreal

Chapters

  • 00:05Title
  • 01:02Preparation of Microneedle for Anterior Intracameral Injection
  • 02:13Induction of Ocular Hypertension
  • 06:51Results: Induction of Ocular Hypertension-Dependent Glaucoma Using the Magnetic Microbead Occlusion Model
  • 08:42Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to induce ocular hypertension in the murine eye that results in the loss of retinal ganglion cells as observed in glaucoma. Magnetic microbeads are injected into the anterior chamber and attracted to the iridocorneal angle using a magnet to block the outflow of aqueous humour.

Tags

Magnetic Microbead Occlusion ModelOcular Hypertension-dependent GlaucomaMiceSurgical ProcedureAnterior ChamberMagnetic Micro-beadsInter-ocular PressureMinimal DamagePuncturing The CorneaLensMicro-needle PreparationMicro-needle PositioningCapillary PullerBohr Silicate Glass CapillaryElliptical ShapeMajor Axis DiameterMinor Axis DiameterMicro-pipette Beveling System

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