Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Chemistry

This content is Open Access.

A Quantitative Glycomics and Proteomics Combined Purification Strategy
 

A Quantitative Glycomics and Proteomics Combined Purification Strategy

Article DOI: 10.3791/53735
March 8th, 2016

Chapters

Summary March 8th, 2016

A high-throughput protocol was developed for combined proteomics and glycomics purification and LC-MS/MS quantification in plasma. Deamidation analysis of N-linked glycosylation motifs was specific to deglycosylated sites. Accurate quantitation of N-glycans was achieved by coupling filter aided N-glycan separation to the individuality normalization when labeling with glycan hydrazide tags strategy.

Transcript

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter