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Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data
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Immunology and Infection
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JoVE Journal Immunology and Infection
Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

DOI:
10.3791/53749-v

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09:09 min

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December 17, 2015

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, , , ,
1Department of Physics and Randall Division of Cell and Molecular Biophysics,King’s College London, 2Academic Department of Rheumatology, Centre for Molecular and Cellular Biology of Inflammation, Division of Immunology, Infection and Inflammatory Disease,King’s College London, 3ARC Centre for Advanced Molecular Imaging, Australian Centre for NanoMedicine,University of New South Wales Australia, 4Departments of Chemistry and Physic,McGill University

Chapters

  • 00:05Title
  • 00:50Microscope Set-up
  • 03:20Cell Imaging
  • 04:44Structured Illumination Microscopy (SIM) Image Reconstruction and Spatio-temporal Image Correlation Spectroscopy (STICS) Analysis
  • 07:12Results: Representative Structured Illumination Data and STICS Output
  • 08:36Conclusion

Summary

Automatic Translation

Automatic Translation

To investigate flow velocities and directionality of filamentous-actin at the T cell immunological synapse, live-cell super-resolution imaging is combined with total internal reflection fluorescence and quantified with spatio-temporal image correlation spectroscopy.

Tags

Keywords: Actin FlowFilamentous ActinF-actinT CellsImmunological SynapseStructured Illumination MicroscopySpatio-temporal Image Correlation SpectroscopySTICSSuper-resolution ImagingRetrograde FlowCentripetal FlowSub-diffractionCell MotilityMolecular DistributionsVesicle DynamicsProtein Clustering

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