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Biology
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単核レベルでのLINE-1レトロ転位の分析
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Biology
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JoVE Journal
Biology
Analysis of LINE-1 Retrotransposition at the Single Nucleus Level
Please note that all translations are automatically generated.
Click here for the English version.
単核レベルでのLINE-1レトロ転位の分析
DOI:
10.3791/53753-v
•
11:52 min
•
April 23, 2016
•
Pasano Bojang
,
Kenneth S. Ramos
2
1
Division of Pulmonary, Allergy, Critical Care and Sleep Medicine
,
University of Arizona College of Medicine
,
2
Center for Applied Genetics and Genomic Medicine
,
University of Arizona College of Medicine
Chapters
00:05
Title
00:44
Chemical Labeling
02:18
Preparing Chromosome Spreads
05:28
Stabilizing, Dehydrating and Hybridization of Spreads with L1-labeled Retrotransposition Probes
07:26
Washing or Addition of Secondary Antibody and Viewing
08:49
Results: Long Interspersed Nuclear Element-1, or LINE-1 Retrotransposition Analysis
10:38
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
ここでは、安定的に合成
LINE-1を
発現
し
たHepG2細胞株の染色体スプレッドの単核レベルでの
LINE-1
レトロ転位を追跡するためにFISH法を採用しています。
Tags
LINE-1 Retrotransposition
Single Nucleus Analysis
FISH
Fluorescence In Situ Hybridization
L-1 Retrotransposition
SNeo Probe
HepG2 Cells
Colcemid
Metaphase Arrest
Trypsin
Cell Culture
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