Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells

The overall goal of this procedure is to grow nasal epithelial cells obtained from children and reprogram them into induced pluripotent stem cells. This method can help answer key questions in the field of aerated disease such as how asthma develops. The main advantage of this technique is that it collects airway epithelial cells from children without stressful procedures.

In culture, these are in a homogeneous population, and serves as an excellent source to generate induced pluripotent stem cells or iPSCs. We first had the idea for this study when we talked to Drs. Ji and Hershey about their study to collect meso-epithelial cells from children to investigate epigenetic variation in asthma and air pollution.