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Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro
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Bioengineering
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JoVE Journal Bioengineering
Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro

Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro

DOI:
10.3791/53823-v

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10:25 min

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March 19, 2016

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, , , ,
1Transfusion Research Center,Belgium Red Cross-Flanders, 2Blood Service,Belgium Red Cross-Flanders, 3Department of Public Health and Primary Care,Catholic University of Leuven, 4Faculty of Medicine and Health Sciences,University of Ghent

Chapters

  • 00:05Title
  • 01:11Flow Chamber Preparation
  • 03:23Preparing the Blood Samples
  • 05:21Perfusion Assay
  • 06:36Data Analysis
  • 07:52Results: Consistency and Utility of the Assay
  • 09:13Conclusion

Summary

Automatic Translation

Automatic Translation

Platelet transfusion and hemostasis was modeled using blood reconstitution and microfluidic flow chambers to investigate the function of blood banking platelets. The data demonstrate the consequences of platelet storage lesion on hemostasis, in vitro.

Tags

Keywords: Microfluidic Flow ChambersReconstituted BloodHemostasisPlatelet TransfusionIn VitroThrombocytopeniaPlatelet ConcentratesThrombosisRheologyCollagenAtherosclerotic PlaqueEndothelial CellsVon Willebrand FactorFibrinogenBiochipBlocking BufferHBSAutomated MicroscopeManifoldPump

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