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Systems Biology of Metabolic Regulation by Estrogen Receptor Signaling in Breast Cancer
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Systems Biology of Metabolic Regulation by Estrogen Receptor Signaling in Breast Cancer

Systems Biology of Metabolic Regulation by Estrogen Receptor Signaling in Breast Cancer

DOI:
10.3791/53832-v

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10:36 min

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March 17, 2016

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1Department of Food Sciences and Human Nutrition,University of Illinois, Urbana-Champaign

Chapters

  • 00:05Title
  • 00:45Preparation of RNA-Seq Samples
  • 02:47Preparation of ChIP-Seq Samples
  • 06:13Metabolomics Assay Sample Collection and Cell Count for Normalization
  • 07:32Results: Metabolic Changes in MCF7 Cells from Estradiol Treatment
  • 09:15Conclusion

Summary

Automatic Translation

Automatic Translation

Integration of data from genome-wide sequencing experiments and metabolomics experiments is a challenge. In this paper we report, for the first time, generation, analysis and integration of transcriptome, cistrome and metabolome data from breast cancer cells treated with estradiol.

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Keywords: Systems BiologyMetabolic RegulationEstrogen Receptor SignalingBreast CancerTranscriptomeSystromeMetabolomeTranscription FactorsMetabolismCell AggressivenessMCF7 CellsRNA IsolationChloroformIsopropanolEthanolDEPC WaterFormaldehydeChromatin Cross-linkingCell Lysis BufferChromatin Fragmentation

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