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Biology

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Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites
 

Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites

Article DOI: 10.3791/53840
March 22nd, 2016

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Summary March 22nd, 2016

We describe a protocol for amplifying retroviral integration sites from the genomic DNA of infected cells, sequencing the amplified virus-host junctions, and then mapping these sequences to a reference genome. We also describe techniques to quantify the distribution of integration sites relative to various genomic annotations using BEDTools.

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