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Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues
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Developmental Biology
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JoVE Journal Developmental Biology
Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues

DOI:
10.3791/53874-v

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13:03 min

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June 03, 2016

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, ,
1INSERM UMR-910, Faculté de Médecine,Université Aix-Marseille, 2Institute of Physiological Chemistry,University Medical Center, Johannes Gutenberg University

Chapters

  • 00:05Title
  • 01:10DNA-protein Cross-linking
  • 02:05Cell Lysis and Chromatin Fragmentation
  • 04:31Chromatin Immunoprecipitation
  • 05:49DNA Elution, Cross-link Reversal and Proteinase K Digestion
  • 07:33DNA Isolation Using DNA-binding Beads
  • 10:05Results: ChIP Protocols Investigate the Epigenetic Regulation of Cardiac Gene Transcription
  • 11:47Conclusion

Summary

Automatic Translation

Automatic Translation

A fine tuning regulation of gene transcription underlies embryonic cell fate decision. Herein, we describe chromatin immunoprecipitation assays used to investigate epigenetic regulation of both cardiac differentiation of stem cells and cardiac development of mouse embryos.

Tags

Keywords: Epigenetic RegulationCardiac DifferentiationEmbryonic Stem CellsChromatin ImmunoprecipitationGene TranscriptionEmbryonic DevelopmentCardiac DevelopmentEpigenetic MarksDNAEmbryoid BodiesCross-linkingCell FixationCell LysisSonicationProtease Inhibitors

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