Journal
/
Developmental Biology
/
Isolation and Characterization of Single Cells from Zebrafish Embryos
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Isolation and Characterization of Single Cells from Zebrafish Embryos

Isolation and Characterization of Single Cells from Zebrafish Embryos

DOI:
10.3791/53877-v

•

09:25 min

•

March 12, 2016

•

, , , ,
1Department of Cell Biology and Physiology,University of North Carolina at Chapel Hill, 2McAllister Heart Institute,University of North Carolina at Chapel Hill, 3Department of Pathology and Laboratory Medicine,University of North Carolina at Chapel Hill

Chapters

  • 00:05Title
  • 01:16Staged Embryo Acquisition and Single Cell Dissociation
  • 05:03Microfluidic Chip Cell Loading
  • 06:55Results: Representative Single Zebrafish Nkx2.5ZsY Positive Cell Analysis
  • 08:16Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a method for isolating single cells from zebrafish embryos, enriching for cells of interest, capturing zebrafish cells in microfluidic based single cell multiplex systems, and assessing gene expression from single cells.

Tags

Keywords: Single Cell IsolationZebrafish EmbryosMicrofluidic-based Single-cell AnalysisGene ExpressionDevelopmental BiologyCell HeterogeneityCell DissociationCell PreparationChorion RemovalDe-yolkingCell WashingCell Resuspension

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/53877?article_type=v

Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle
check_url/53877?article_type=v

Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles
check_url/53877?article_type=v

Affinity Labeling Detection of Endogenous Receptors from Zebrafish Embryos
check_url/53877?article_type=v

Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta
check_url/53877?article_type=v

Single-cell Photoconversion in Living Intact Zebrafish
check_url/53877?article_type=v

Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients
check_url/53877?article_type=v

Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants
check_url/53877?article_type=v

Isolation, Culture, Characterization, and Differentiation of Human Muscle Progenitor Cells from the Skeletal Muscle Biopsy Procedure
check_url/53877?article_type=v

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos
check_url/53877?article_type=v

Generation of Naïve Blastoderm Explants from Zebrafish Embryos

Read Article