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Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma

Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma

DOI:
10.3791/53884-v

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09:25 min

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October 14, 2016

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, , , , , , , , ,
1Department of Surgery and Translational Medicine (DCMT),University of Florence, 2Neurofarba Department,University of Florence, 3Department of Traumatology and General Orthopedics,Azienda Ospedaliera Universitaria Careggi

Chapters

  • 00:05Title
  • 01:20Establishment of an Osteosarcoma Cell Line and the Sarcosphere Assay
  • 03:12Isolation of Sarcospheres
  • 05:48In Vitro Analysis to Characterize the Osteosarcoma Cancer Stem Cells
  • 06:24Results: Establishing a Verified Culture of Cancer Stem Cells
  • 07:55Conclusion

Summary

Automatic Translation

Automatic Translation

The presence of cancer stem cells (CSCs) in bone sarcomas has recently been linked to their pathogenesis. In this article, we present the isolation of CSCs from primary cell cultures obtained from human biopsies of conventional osteosarcoma (OS) using the ability of CSCs to grow under nonadherent conditions.

Tags

Keywords: Cancer Stem CellsOsteosarcomaSarcosphereIn VitroCell CultureCell IsolationCell SuspensionGrowth FactorsEGFBFGFUltra-low Attachment PlatesHemocytometerPhase Contrast Microscopy

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