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Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells (ASCs)
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Developmental Biology
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JoVE Journal Developmental Biology
Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells (ASCs)

Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells (ASCs)

DOI:
10.3791/53890-v

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08:52 min

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August 11, 2016

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,
1Department of Internal Medicine, Division of Metabolism, Endocrinology and Diabetes,Biointerfaces Institute, University of Michigan

Chapters

  • 00:05Title
  • 00:45Subcutaneous (SQ) and Visceral (VIS) Fat Pad Isolation
  • 02:35Fat Pad Collagenase Digestion
  • 04:20Magnetic Cell Separation
  • 06:31Results: Fat Depot-dependent Expression of Collagenolytic Matrix Metalloproteinases (MMPs)
  • 07:52Conclusion

Summary

Automatic Translation

Automatic Translation

We present the techniques required to isolate the stromal vascular fraction (SVF) from mouse inguinal (subcutaneous) and perigonadal (visceral) adipose tissue depots to assess their gene expression and collagenolytic activity. This method includes the enrichment of Sca1high adipose-derived stem cells (ASCs) using immunomagnetic cell separation.

Tags

Keywords: Immunomagnetic SeparationSca1high Adipose-derived Stem CellsFat Depot-specificMesenchymal Stem CellsSubcutaneous FatVisceral FatCollagenaseCell Sorting

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