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JoVE Journal
Immunology and Infection

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In Vitro Disassembly of Influenza A Virus Capsids by Gradient Centrifugation
 

In Vitro Disassembly of Influenza A Virus Capsids by Gradient Centrifugation

Article doi: 10.3791/53909
March 27th, 2016

Summary March 27th, 2016

Disassembly of influenza A virus cores during virus entry into host cells is a multistep process. We describe an in vitro method to analyze the early stages of viral uncoating. In this approach, velocity gradient centrifugation is used to biochemically dissect the steps that initiate uncoating under defined conditions.

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