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March 27, 2016
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Summary
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Disassembly of influenza A virus cores during virus entry into host cells is a multistep process. We describe an in vitro method to analyze the early stages of viral uncoating. In this approach, velocity gradient centrifugation is used to biochemically dissect the steps that initiate uncoating under defined conditions.
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Cite this Article
Stauffer, S., Nebioglu, F., Helenius, A. In Vitro Disassembly of Influenza A Virus Capsids by Gradient Centrifugation. J. Vis. Exp. (109), e53909, doi:10.3791/53909 (2016).
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