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In Vitro Disassembly of Influenza A Virus Capsids by Gradient Centrifugation
<em>In Vitro</em> Disassembly of Influenza A Virus Capsids by Gradient Centrifugation
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
In Vitro Disassembly of Influenza A Virus Capsids by Gradient Centrifugation

In Vitro Disassembly of Influenza A Virus Capsids by Gradient Centrifugation

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07:24 min

March 27, 2016

DOI:

07:24 min
March 27, 2016

8959 Views
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Summary

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Disassembly of influenza A virus cores during virus entry into host cells is a multistep process. We describe an in vitro method to analyze the early stages of viral uncoating. In this approach, velocity gradient centrifugation is used to biochemically dissect the steps that initiate uncoating under defined conditions.

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