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Studying Wnt Signaling During Patterning of Conducting Airways
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Studying Wnt Signaling During Patterning of Conducting Airways
DOI:

13:00 min

October 16, 2016

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Chapters

  • 00:05Title
  • 01:20Whole Mount X-galactosidase Staining
  • 05:23Lectin Staining
  • 06:18Whole Mount Immunofluorescence Staining and Confocal Microscopy
  • 07:43Cell Proliferation
  • 09:37Results: Wnt Signaling and Developing Tracheal and Lung Tissue
  • 11:28Conclusion

Summary

Automatic Translation

The use of reporter mice coupled to whole mount and section staining, microscopy and in vivo assays facilitates the analysis of mechanisms underlying the normal patterning of the respiratory tract. Here we describe how these techniques contributed to the analysis of Wnt signaling during tracheal development.

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