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Using a Laminating Technique to Perform Confocal Microscopy of the Human Sclera
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Using a Laminating Technique to Perform Confocal Microscopy of the Human Sclera

Using a Laminating Technique to Perform Confocal Microscopy of the Human Sclera

DOI:
10.3791/53920-v

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07:22 min

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May 06, 2016

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, , ,
1Department of Ophthalmology,University of Cologne

Chapters

  • 00:05Title
  • 00:43Experimental Preparation
  • 01:30Preparation of the Sclera
  • 03:58Immunohistochemistry of Scleral Layers
  • 05:56Results: Confocal Microscopy of Laminated Human Sclera
  • 06:47Conclusion

Summary

Automatic Translation

Automatic Translation

Human sclera tissue is mainly collagen; therefore, it is not easily usable for immunohistochemistry. To achieve the goal of performing immunohistochemistry for confocal microscopy of scleral tissue, a laminating technique was used.

Tags

Keywords: Confocal MicroscopyHuman ScleraImmune Cell DistributionVascular StatusLaminating TechniqueScleral SamplesPBSBSAAntibodiesMicro-dissection

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