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Biology

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Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry
 

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

Article doi: 10.3791/53924
May 17th, 2016

Summary May 17th, 2016

MyoD is a myogenic transcription factor with a strong capacity to induce myogenic transdifferentiation of many fully differentiated non-muscle cell lines. The epigenetic mechanisms involved in this transdifferentiation are largely unknown. Here we describe a double-affinity purification method followed by mass spectrometry to exhaustively characterize MyoD partners.

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