Journal
/
Medicine
/
Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization

DOI:
10.3791/53928-v

•

06:01 min

•

June 07, 2016

•

, ,
1Department of Biological Science, Center for Colon Cancer Research,University of South Carolina, 2Department of Pathology,Chonnam National University Medical School

Chapters

  • 00:05Title
  • 00:37Tissue Preparation
  • 02:02Hybridization
  • 03:08Stringency Wash and Blocking
  • 03:55Primary Antibody Incubation and Development
  • 05:14Results: Stronger miRNA-489 Expression in Normal Tissues
  • 05:37Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a protocol to detect miRNA expression in breast cancer patient samples using miRNA in situ hybridization.

Tags

Keywords: MiRNAIn Situ HybridizationBreast CancerProbe HybridizationWash Steps

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/53928?article_type=v

Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy
check_url/53928?article_type=v

Multi-modal Imaging of Angiogenesis in a Nude Rat Model of Breast Cancer Bone Metastasis Using Magnetic Resonance Imaging, Volumetric Computed Tomography and Ultrasound
check_url/53928?article_type=v

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
check_url/53928?article_type=v

Quantification of Breast Cancer Cell Invasiveness Using a Three-dimensional (3D) Model
check_url/53928?article_type=v

Carotid Artery Infusions for Pharmacokinetic and Pharmacodynamic Analysis of Taxanes in Mice
check_url/53928?article_type=v

Mammosphere Formation Assay from Human Breast Cancer Tissues and Cell Lines
check_url/53928?article_type=v

In Vivo and Ex Vivo Approaches to Study Ovarian Cancer Metastatic Colonization of Milky Spot Structures in Peritoneal Adipose
check_url/53928?article_type=v

Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies
check_url/53928?article_type=v

miRNA Expression Analyses in Prostate Cancer Clinical Tissues
check_url/53928?article_type=v

Systems Biology of Metabolic Regulation by Estrogen Receptor Signaling in Breast Cancer

Read Article