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Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin
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Developmental Biology
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JoVE Journal Developmental Biology
Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

DOI:
10.3791/53931-v

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06:51 min

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April 29, 2016

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, , , , ,
1Department of Biology and Lokey Center, Laboratory of Stem Cell Biology and Regenerative Medicine,Technion Israel Institute of Technology, 2Strang Laboratory of Apoptosis and Cancer Biology,Howard Hughes Medical Institute, The Rockefeller University

Chapters

  • 00:05Title
  • 00:44Hair Follicle Isolation from Adult Epidermis
  • 02:30Hair Follicle Cell Suspension Preparation
  • 05:22Results: Representative Hair Follicle Cell Profiling
  • 06:22Conclusion

Summary

Automatic Translation

Automatic Translation

An ideal model for studying adult stem cell biology is the mouse hair follicle. Here we present a protocol for isolating different populations of hair follicles stem cells and epidermal keratinocytes, employing enzymatic digestion of mouse dorsal skin followed by FACS analysis.

Tags

Keywords: Hair Follicle Stem CellsEpidermal KeratinocytesDorsal Mouse SkinCell IsolationCell PopulationHair Follicle CycleSkin DissectionTrypsin DigestionHair Follicle Removal

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