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12:37 min
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April 14, 2016
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Summary
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Descriviamo un metodo efficiente, robusto e redditizio per l'estrazione degli acidi nucleici da tamponi per la caratterizzazione delle comunità batteriche che utilizzano 16S rRNA gene amplicone sequenziamento. Il metodo consente un approccio di elaborazione comune per diversi tipi di campioni ed accoglie una serie di processi analitici valle.
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Cite this Article
Anahtar, M. N., Bowman, B. A., Kwon, D. S. Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization. J. Vis. Exp. (110), e53939, doi:10.3791/53939 (2016).
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