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더블 형광 결합<em> 현장에서</em> 마우스 뇌 섹션에서 세 가지 유전자의 발현의 검출을위한 Immunolabelling와 하이브리드
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections
DOI:

09:23 min

March 26, 2016

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Chapters

  • 00:05Title
  • 00:42Overview of Double Fluoresence In Situ Hybridization Followed by Immunostaining
  • 01:44RNA Probe Synthesis
  • 04:12Cryosectioning and Hybridization
  • 05:33Visualization of the FITC Probe and the DIG Probe
  • 07:23Immunohistochemistry and Mounting
  • 08:16Results: Expression of Aspa in Oligodendrocytes in the Cortex and the Corpus Callosum
  • 08:45Conclusion

Summary

Automatic Translation

Localizing gene expression to specific cell types can be challenging due to the lack of specific antibodies. Here we describe a protocol for simultaneous triple detection of gene expression by combining double fluorescence RNA in situ hybridization with immunostaining.

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