Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

The overall goal of this video is to provide an integrated set of methods to identify and experimentally validate geneomic regulatory elements so called enhancers. This method can help answer key questions in the transcription and regulation of biologic events, including enhancer selection and usage, using cell differentiation, or in response to external stimuli. The main advantage to the presented work-flow is that a protocol can be easily adapted and used in any cellular model systems.

I will demonstrate the steps of the procedure. To begin, choose a candidate enhancer based on pre-existing chip seq analysis. Use a genome browser to view the results of the desired chip seq experiment, and identify binding sites in the proximity of the gene of interest.