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Simultaneous Assessment of Cardiomyocyte DNA Synthesis and Ploidy: A Method to Assist Quantification of Cardiomyocyte Regeneration and Turnover
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Simultaneous Assessment of Cardiomyocyte DNA Synthesis and Ploidy: A Method to Assist Quantification of Cardiomyocyte Regeneration and Turnover

Simultaneous Assessment of Cardiomyocyte DNA Synthesis and Ploidy: A Method to Assist Quantification of Cardiomyocyte Regeneration and Turnover

DOI:
10.3791/53979-v

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08:03 min

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May 23, 2016

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1Institute of Genetic Medicine, International Centre for Life,Newcastle University

Chapters

  • 00:05Title
  • 00:48Heart Harvest
  • 02:13Nuclei Dissociation and Cardiomyocyte Nuclei Labeling
  • 04:51EdU Incorporation Detection
  • 06:05Results: EdU Labeled Cardiomyocyte Quantification in WT and Mdx Hearts
  • 07:03Conclusion

Summary

Automatic Translation

Automatic Translation

Quantification of cardiomyocyte turnover is challenging. The protocol described here makes an important contribution to this challenge by enabling accurate and sensitive quantification of neo-cardiomyocyte nuclei generation and nuclei ploidy.

Tags

Keywords: CardiomyocyteDNA SynthesisPloidyRegenerationTurnoverFlow CytometryNucleoside LabelingEdUPolyploidizationCell LysisHomogenizationSucrose Gradient

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