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Genetics

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siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts
 

siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts

Article doi: 10.3791/53995
September 20th, 2016

Summary September 20th, 2016

This protocol describes a method for efficiently transfecting siRNA in freshly isolated human villous cytotrophoblasts using microporation and identifying DNA-protein complexes in these cells. Transfected cells can be monitored by Western blot and EMSA analyses during the 4-day culture time.

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