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JoVE Journal Developmental Biology
In Vitro Colony saggi per Caratterizzazione Tri-potenti cellule progenitrici isolato dal adulti murini Pancreas
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In Vitro Colony saggi per Caratterizzazione Tri-potenti cellule progenitrici isolato dal adulti murini Pancreas

Article DOI: 10.3791/54016-v • 09:31 min • June 10th, 2016
June 10th, 2016 •
*1,2, *1, 1, 1, 1, 1, 1, 3, 1
1Diabetes and Metabolism Research Institute, Beckman Research Institute of City of Hope, 2Irell & Manella Graduate School of Biological Sciences, Beckman Research Institute of City of Hope, 3Division of Chemistry and Chemical Engineering, California Institute of Technology
* These authors contributed equally

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Nei saggi di colonie in vitro per rilevare auto-rinnovamento e la differenziazione delle cellule progenitrici isolate dal pancreas di topo adulti sono messo a punto. In questi saggi, progenitori pancreatici danno origine a colonie di cellule nello spazio 3-dimensionale in terreno semisolido contenente metilcellulosa. I protocolli per la gestione di singole cellule e caratterizzazione delle singole colonie sono descritti.

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Biologia dello Sviluppo Numero 112 unità formanti colonie pancreatici cellule progenitrici cellule fluorescenza-attivato (FACS) analizza singola colonia analisi singola cellula proteine ​​della matrice extracellulare laminina idrogel metilcellulosa mezzo semisolido immunocolorazione tutto il montaggio microfluidica PCR topi adulti
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