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09:44 min
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June 13, 2016
DOI:
1London Regional Cancer Program,London Health Sciences Centre, 2Department of Anatomy & Cell Biology, Schulich School of Medicine & Dentistry,Western University, 3Department of Biochemistry, Schulich School of Medicine & Dentistry,Western University, 4Department of Oncology, Schulich School of Medicine & Dentistry,Western University, 5Lawson Health Research Institute
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This manuscript describes an ex vivo model system comprised of organ-conditioned media derived from the lymph node, bone, lung, and brain of mice. This model system can be used to identify and study organ-derived soluble factors and their effects on the organ tropism and metastatic behavior of cancer cells.
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Piaseczny, M. M., Pio, G. M., Chu, J. E., Xia, Y., Nguyen, K., Goodale, D., Allan, A. Generation of Organ-conditioned Media and Applications for Studying Organ-specific Influences on Breast Cancer Metastatic Behavior. J. Vis. Exp. (112), e54037, doi:10.3791/54037 (2016).
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