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Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
 

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Article DOI: 10.3791/54059
August 8th, 2016

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Summary August 8th, 2016

This work presents a method of high-throughput screening using a universal genetic enzyme screening system that can be theoretically applied to over 200 enzymes. Here, the single screening system identifies three different enzymes (lipase, cellulase, and alkaline phosphatase) by simply changing the substrate used (p-nitrophenyl acetate, p-nitrophenyl-β-D-cellobioside, and phenyl phosphate).

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