Journal
/
Medicine
/
Modeling Myotonic Dystrophy 1 in C2C12 Myoblast Cells
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Modeling Myotonic Dystrophy 1 in C2C12 Myoblast Cells

Modeling Myotonic Dystrophy 1 in C2C12 Myoblast Cells

DOI:
10.3791/54078-v

•

09:39 min

•

July 29, 2016

•

, , , , ,
1Department of Biology and Biochemistry,University of Houston, 2Department of Economics,University of Houston, 3Department of Cardiology,The First Affiliated Hospital of Nanchang University

Chapters

  • 00:05Title
  • 00:40C2C12 Transfection
  • 02:06C2C12 Retroviral Transduction
  • 04:44C2C12 Lentiviral Transduction
  • 06:46Results: The Effects of Celf1 in Myoblast Differentiation
  • 08:53Conclusion

Summary

Automatic Translation

Automatic Translation

In this protocol, we present the procedures in establishing myotonic dystrophy 1 myoblast models, including optimized C2C12 cell maintenance, gene transfection/transduction, and myocyte differentiation.

Tags

Keywords: C2C12 MyoblastsMyotonic DystrophyM1 Cell ModelGene ManipulationTransfectionTransductionRetrovirusPackaging CellsSelection AntibioticCell CultureCell MorphologyCytotoxicity

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/54078?article_type=v

Modeling Stroke in Mice – Middle Cerebral Artery Occlusion with the Filament Model
check_url/54078?article_type=v

Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase
check_url/54078?article_type=v

Modeling Spontaneous Metastatic Renal Cell Carcinoma (mRCC) in Mice Following Nephrectomy
check_url/54078?article_type=v

Modeling Stroke in Mice: Permanent Coagulation of the Distal Middle Cerebral Artery
check_url/54078?article_type=v

Generation of Induced Pluripotent Stem Cells from Muscular Dystrophy Patients: Efficient Integration-free Reprogramming of Urine Derived Cells
check_url/54078?article_type=v

Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies
check_url/54078?article_type=v

Modeling Chemotherapy Resistant Leukemia In Vitro
check_url/54078?article_type=v

Multi-exon Skipping Using Cocktail Antisense Oligonucleotides in the Canine X-linked Muscular Dystrophy
check_url/54078?article_type=v

Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells
check_url/54078?article_type=v

Evaluation of Exon Inclusion Induced by Splice Switching Antisense Oligonucleotides in SMA Patient Fibroblasts

Read Article