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Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber
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Bioengineering
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JoVE Journal Bioengineering
Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber

Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber

DOI:
10.3791/54099-v

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09:55 min

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May 30, 2016

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, , , ,
1O’Brien Institute Department,St Vincent’s Institute of Medical Research, 2Department of Surgery,University of Melbourne, 3Faculty of Health Sciences,Australia Catholic University

Chapters

  • 00:05Title
  • 00:53Preparing the Animal for Surgery
  • 01:36Harvest of Femoral Vein Graft
  • 03:59Creating an Arteriovenous Loop and Implanting the Chamber
  • 07:06Flow-through Pedicle Chamber (Two Chambers per Animal)
  • 07:52Results: Uses for the Implanted Chambers
  • 09:04Conclusion

Summary

Automatic Translation

Automatic Translation

This is a guideline for constructing in vivo vascularized tissue using a microsurgical arteriovenous loop or a flow-through pedicle configuration inside a tissue engineering chamber. The vascularized tissues generated can be employed for organ regeneration and replacement of tissue defects, as well as for drug testing and disease modeling.

Tags

VascularizationBlood SupplyReconstructive SurgeryOrgan ReplacementOrgan RepairIntrinsic VascularizationAnesthesiaSurgical ProcedureInguinal Fat PadFemoral VesselsEpigastric VeinPerivascular SheathMicromanipulationThrombosis

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