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Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes
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Chapters
- 00:05Title
- 01:17Preparation of Cationic Liposome-siRNA-Peptide Complexes (LSPCs)
- 03:25Preparation of Peptide Addressed Liposome Encapsulated Therapeutic siRNA (PALETS)
- 06:03Tail-vein Injection of LSPCs or PALETS
- 07:09Analysis of Protein Expression by Flow Cytometry
- 09:14Results: Representative Flow Cytometric Analysis of Brian Cell Suspensions 24 hr After Treatment with LSPCs
- 09:56Conclusion
The goal of this protocol is to use cationic/anionic liposomes with a neuro-targeting peptide as a CNS delivery system to enable siRNA to cross the BBB. The optimization of a delivery system for treatments, like siRNA, would allow for more treatment options for prion and other neurodegenerative diseases.
