Covalent Binding of Antibodies to Cellulose Paper Discs and Their Applications in Naked-eye Colorimetric Immunoassays

The overall goal of this methodology is to stabilize antibodies and proteins on a cellulose matrix to facilitate development of a naked-eye colorimetric immunoassay which can be useful for point-of-care diagnostics. This method can help answer key questions in the point-of-care diagnostic field. The main advantage of this technique is that it helps stabilize protein on cellulose which allows the development of low-cost paper-based diagnostic assays.

Demonstrating the procedure will be Gloria Chang, a student from my laboratory. To begin, prepare one piece of square paper with a dimension of one centimeter by one centimeter. Also prepare 100 paper discs made from Whatman grade number one cellulose paper with a diameter of 6.0 millimeters using a hole punch.