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Methods for Acute and Subacute Murine Hindlimb Ischemia
 

Methods for Acute and Subacute Murine Hindlimb Ischemia

Article DOI: 10.3791/54166
June 21st, 2016

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Summary June 21st, 2016

Surgical induction of hindlimb ischemia in the mouse is useful to examine angiogenesis, however this is compromised in certain inbred mouse strains that display marked ischemia-induced tissue necrosis. Methods are described to induce subacute limb ischemia using ameroid constrictors to circumvent this problem through the induction of gradual arterial occlusion.

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