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JoVE Journal
Developmental Biology

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Generation of Parabiotic Zebrafish Embryos by Surgical Fusion of Developing Blastulae
 

Generation of Parabiotic Zebrafish Embryos by Surgical Fusion of Developing Blastulae

Article doi: 10.3791/54168
June 11th, 2016

Summary June 11th, 2016

This protocol provides step-by-step instruction on how to generate parabiotic zebrafish embryos of different genetic backgrounds. When combined with the unparalleled imaging capabilities of the zebrafish embryo, this method provides a uniquely powerful means to investigate cell-autonomous versus non-cell-autonomous functions for candidate genes of interest.

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