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Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery
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1Department of Orthopaedic Surgery, Yong Loo Lin School of Medicine,National University of Singapore, 2Centre for Research in Medical Devices (CÚRAM),National University of Ireland Galway, 3Department of Bioengineering, Faculty of Engineering,National University of Singapore, 4Tissue Engineering Program,National University of Singapore, 5Department of Orthopaedic Surgery and the Orthopaedic Hospital Research Center,University of California, Los Angeles
Chapters
- 00:05Title
- 00:49Alginate Microbead Fabrication
- 03:31Size Measurement and Sterilization of Alginate Microbeads
- 05:29Protamine and Heparin Coating
- 06:38BMP-2 and NELL-1 Uptake and Release
- 07:43In Vitro Bioactivity of NELL-1 and BMP-2 & NELL-1 Loading into PEC Scaffold
- 09:57Results: Analysis of the Polyelectrolyte Complex for Growth Factor Uptake Fabrication Process
- 11:28Conclusion
Self-assembled polyelectrolyte complexes (PEC) fabricated from heparin and protamine were deposited on alginate beads to entrap and regulate the release of osteogenic growth factors. This delivery strategy enables a 20-fold reduction of BMP-2 dose in spinal fusion applications. This article illustrates the benefits and fabrication of PECs.
