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Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue

Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue

DOI:
10.3791/54204-v

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12:40 min

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June 06, 2016

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, , , , ,
1UQ Centre for Clinical Research,The University of Queensland, 2Translational Research Institute,Queensland University of Technology, 3Translational Research Institute,Mater Medical Research – University of Queensland

Chapters

  • 00:00Title
  • 01:00Methodologies
  • 02:20Introduction
  • 03:12Tissues to be Harvested
  • 03:53Preparation of the Placenta and the Placental Anatomy
  • 04:40Dissection of the Decidua (D) Tissue
  • 05:40Dissection of the Chorionic Plate (CP) Tissue and Dissection of the Chorionic Villi (CV) Tissue
  • 06:36Mincing and Enzymatic Digestion of the Placental Tissue
  • 07:25Collection of Mononuclear Cells and Plating into Flasks
  • 09:33Post Isolation – Cell Growth and Expansion
  • 09:57Representative Results: Culture MSC and Assay Cells for MSC Characteristics
  • 12:07Conclusion

Summary

Automatic Translation

Automatic Translation

Herein we describe methods for the dissection of fetal and maternal tissues from human term placenta, followed by isolation and expansion of mesenchymal stem/stromal cells (MSC) from these tissues.

Tags

Keywords: Mesenchymal Stem CellsMSC IsolationCell CultureFetal-derived CellsMaternal CellsDeciduaChorionic VilliChorionic Plate

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