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Biology
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Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells
 

Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells

Article doi: 10.3791/54239
April 4th, 2016

Summary April 4th, 2016

This paper presents a sensitive method called Circle-Seq for purifying extrachromosomal circular DNA (eccDNA). The method encompasses column purification, removal of remaining linear chromosomal DNA, rolling-circle amplification and high-throughput sequencing. Circle-Seq is applicable to genome-scale screening of eukaryotic eccDNA and studying genome instability and copy-number variation.

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