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High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells
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Biology
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JoVE Journal Biology
High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells

High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells

DOI:
10.3791/54265-v

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10:25 min

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August 13, 2016

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, , ,
1Department of Biological Sciences,University of the Sciences in Philadelphia

Chapters

  • 00:05Title
  • 01:03Coverslip Preparation Prior to Human Umbilical Vein Endothelial Cell (HUVEC) Culture
  • 02:06cDNA Transfection and HUVEC Culture on Coverslip
  • 03:36Preparation of Specimen for Imaging
  • 05:14Cell Migration Assay
  • 07:24Representative Imaging Results
  • 09:41Conclusion

Summary

Automatic Translation

Automatic Translation

Protocols for Human Umbilical Vein Endothelial Cell (HUVEC) culture, transient transfection of fluorescently-labeled markers of microtubule growth, live-cell imaging and automated analysis of interphase microtubule growth dynamics are detailed.

Tags

Keywords: Time-lapse ImagingMicrotubule DynamicsHuman Umbilical Vein Endothelial CellsCell CultureCytoskeletonCell MigrationECMCell MotilityAngiogenesisAdherent CellFibronectinElectroporationCDNA Transfection

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