A Detailed Protocol for Characterizing the Murine C1498 Cell Line and its Associated Leukemia Mouse Model

Alexia Mopin; Virginie Driss; Carine Brinster

doi:10.3791/54270

Journal

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Cancer Research

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A Detailed Protocol for Characterizing the Murine C1498 Cell Line and its Associated Leukemia Mouse Model

JoVE Journal
Cancer Research

This content is Free Access.

JoVE Journal Cancer Research

A Detailed Protocol for Characterizing the Murine C1498 Cell Line and its Associated Leukemia Mouse Model

20,170 Views

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08:00 min

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October 14, 2016

DOI:

10.3791/54270-v

DOI:

10.3791/54270-v

•

08:00 min

October 14, 2016

•

20158 Views

Alexia Mopin¹, Virginie Driss¹, Carine Brinster^1,2

¹Institut pour la Recherche sur le Cancer de Lille (IRCL),INSERM, UMR-S-1172, Centre de Recherche Jean-Pierre Aubert (JPARC)²Université de Lille

Summary

This manuscript provides a technical procedure that can be used to characterize C1498 cell cultures in vitro and the acute leukemia induced in mice after their injection. Phenotypic and functional analyses are performed using flow cytometry, immunofluorescence microscopy, cytochemistry and May-Grünwald Giemsa staining.