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A Detailed Protocol for Characterizing the Murine C1498 Cell Line and its Associated Leukemia Mouse Model
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
A Detailed Protocol for Characterizing the Murine C1498 Cell Line and its Associated Leukemia Mouse Model

A Detailed Protocol for Characterizing the Murine C1498 Cell Line and its Associated Leukemia Mouse Model

DOI:
10.3791/54270-v

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08:00 min

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October 14, 2016

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, ,
1Institut pour la Recherche sur le Cancer de Lille (IRCL),INSERM, UMR-S-1172, Centre de Recherche Jean-Pierre Aubert (JPARC), 2Université de Lille

Chapters

  • 00:05Title
  • 00:53Intravenous C1498 Injection and Retro-orbital Blood Collection
  • 02:15Blood Mononuclear Cells Isolation
  • 03:11Bone Marrow Cells Isolation
  • 04:41Bone Marrow Cells Preparation on Slides and May-Grünwald Giemsa staining
  • 06:06Representative Results: C1498 Acute Myeloid Leukemia Characterization
  • 07:14Conclusion

Summary

Automatic Translation

Automatic Translation

This manuscript provides a technical procedure that can be used to characterize C1498 cell cultures in vitro and the acute leukemia induced in mice after their injection. Phenotypic and functional analyses are performed using flow cytometry, immunofluorescence microscopy, cytochemistry and May-Grünwald Giemsa staining.

Tags

Keywords: C1498 CellsMurine Myelomonocytic LeukemiaAcute LeukemiaAnimal ModelCongenic C57 Black 6 MouseTail Vein InjectionOrbital Sinus Blood DrawMononuclear Cell IsolationFlow CytometryBone Marrow IsolationLeukemia DevelopmentImmune DeficiencyChemo And Immunotherapeutic Agents

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