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JoVE Journal
Biology

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Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets
 

Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets

Article DOI: 10.3791/54278
August 13th, 2016

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Summary August 13th, 2016

The assembly and positioning of the mitotic spindle depend on the combined forces generated by microtubule dynamics, motor proteins and cross-linkers. Here we present our recently developed methods in which the geometrical confinement of spherical emulsion droplets is used for the bottom-up reconstitution of basic mitotic spindles.

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