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Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution
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Developmental Biology
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JoVE Journal Developmental Biology
Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution

Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution

DOI:
10.3791/54303-v

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07:30 min

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October 07, 2016

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, , , , , , , ,
1Department of Molecular and Cellular Physiology,Albany Medical College, 2Department of Neuroscience and Experimental Therapeutics,Albany Medical College

Chapters

  • 00:05Title
  • 01:06Whole Mount Staining
  • 03:00Scar Tissue Clearing Method
  • 04:27CUBIC Tissue Clearing Method
  • 05:42Results: Imaging the Cleared Embryonic Heart
  • 07:02Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a protocol to volumetrically image fluorescent protein labeled cells deep inside intact embryonic and postnatal hearts. Utilizing tissue-clearing methods in combination with whole mount staining, single fluorescent protein-labeled cells inside an embryonic or postnatal heart can be imaged clearly and accurately.

Tags

Keywords: Single-cell ResolutionCardiac DevelopmentFluorescent ProteinPECAMAlexa Fluor 647DAPICUBICSCALEEmbryonic HeartPostnatal HeartGene FunctionProtein FunctionImagingConfocal Microscopy

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