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JoVE Journal
Developmental Biology

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Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution
 

Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution

Article doi: 10.3791/54303
October 7th, 2016

Summary October 7th, 2016

We describe a protocol to volumetrically image fluorescent protein labeled cells deep inside intact embryonic and postnatal hearts. Utilizing tissue-clearing methods in combination with whole mount staining, single fluorescent protein-labeled cells inside an embryonic or postnatal heart can be imaged clearly and accurately.

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