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A Method of Targeted Cell Isolation via Glass Surface Functionalization

Article DOI: 10.3791/54315-v • 10:40 min • September 20th, 2016
September 20th, 2016 •
1, 2, 1, 3, 1
1Department of Bioengineering, University of Illinois at Urbana-Champaign, 2Department of Liberal Arts & Sciences, University of Illinois at Urbana-Champaign, 3Department of Biomedical Engineering, Illinois Institute of Technology

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Summary
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This protocol describes customizable surface functionalization of the desthiobiotin, streptavidin, and APTES system in order to isolate specific cell types of interest. In addition, this manuscript covers the applications, optimization, and verification of this process.

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Targeted Cell Isolation Glass Surface Functionalization Capture And Release Of Cells Tumor Angiogenesis Cell Biomarkers Drug Resistance Customizable Technique Antibody Specific Cancer Angiogenesis Personalized Treatment Regimens Reversible Release Mechanism Desthiobiotin Adivin Family Oxygen Plasma Machine 3-Aminopropyl Triethoxysilane (APTES) Solution Ethanol Conical Tube Platform Shaker
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