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JoVE Journal
Immunology and Infection

This content is Open Access.

High-throughput Gene Tagging in Trypanosoma brucei
 

High-throughput Gene Tagging in Trypanosoma brucei

Article DOI: 10.3791/54342
August 12th, 2016

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Summary August 12th, 2016

Addition of a tag to a protein is a powerful way of gaining insight into its function. Here, we describe a protocol to endogenously tag hundreds of Trypanosoma brucei proteins in parallel such that genome scale tagging is achievable.

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