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Journal / Environment / Ekstraktion og analyse af Mikrobiel Phospholipid…
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Ekstraktion og analyse af Mikrobiel Phospholipid fedtsyrer i Jordbunden
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Ekstraktion og analyse af Mikrobiel Phospholipid fedtsyrer i Jordbunden

Article DOI: 10.3791/54360-v • 10:03 min • August 26th, 2016
August 26th, 2016 •
1, 2, 1, 3, 4, 5
1Department of Renewable Resources, University of Alberta, 2Department of Science, Augustana Faculty, University of Alberta, 3Laboratoire Génie Civil et géo-Environnement, Université de Lille, 4Department of Earth and Environmental Sciences, Mount Royal University, 5Forest Ecology & Production, Great Lakes Forestry Centre, Natural Resources Canada

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Phospholipid fedtsyrer giver oplysninger om strukturen af ​​jordens mikrobielle samfund. Vi præsenterer fremgangsmåder til ekstraktion fra jordprøver med en enfaset chloroform-blanding, fraktionering af ekstraherede lipider ved anvendelse af fastfase ekstraktionskolonner, og methanolyse for at fremstille fedtsyremethylestere, som analyseres ved kapillær gaschromatografi.

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Environmental Sciences mikrobielle samfund struktur phospholipid fedtsyrer PLFA jordens mikroorganismer jord biologi mikrobiel økologi ændringer i arealanvendelse arealforvaltning
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