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Generation of Induced Pluripotent Stem Cells from Human Melanoma Tumor-infiltrating Lymphocytes

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Developmental Biology

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JoVE Journal Developmental Biology

Generation of Induced Pluripotent Stem Cells from Human Melanoma Tumor-infiltrating Lymphocytes

Generation of Induced Pluripotent Stem Cells from Human Melanoma Tumor-infiltrating Lymphocytes

DOI:

10.3791/54375-v

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10:03 min

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November 11, 2016

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Hidehito Saito², Kumiko Iwabuchi, Noemi Fusaki⁵, Fumito Ito⁶

¹Department of Surgery,University of Michigan, ²Department of Biochemistry II,Kanazawa Medical University, ³Center for Immunotherapy,Roswell Park Cancer Institute, ⁴DNAVEC Corporation, ⁵Department of Ophthalmology,Keio University School of Medicine, ⁶Department of Surgical Oncology,Roswell Park Cancer Institute

Chapters

00:05Title
01:17Tumor-infultrating Lymphocyte (TIL) Isolation and Culture
03:58Mitomycin-C-treated SNL Feeder Cell Plate Preparation
05:28Sendai Virus (SeV) Vector Induced Pluripotent Stem Cell (iPSC) Generation
07:55Results: Representative TIL-derived iPSC Generation
09:18Conclusion

Summary

Automatic Translation

The goal of this protocol is to show the protocol for reprogramming melanoma tumor-infiltrating lymphocytes into induced pluripotent stem cells.

Tags

Keywords: Induced Pluripotent Stem Cells IPSCs Melanoma Tumor-infiltrating Lymphocytes Cancer Immunotherapy T Cell Receptor Repertoire Tumor Microenvironment Tumor Dissociation Density Gradient Separation T-cell Medium IL-2

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